Glutaredoxin 1 Deficiency Leads to Microneme Protein-Mediated Growth Defects in Neospora caninum.
Identifieur interne : 000071 ( Main/Exploration ); précédent : 000070; suivant : 000072Glutaredoxin 1 Deficiency Leads to Microneme Protein-Mediated Growth Defects in Neospora caninum.
Auteurs : Xingju Song [République populaire de Chine] ; Xu Yang [République populaire de Chine] ; Yangfei Xue [République populaire de Chine] ; Congshan Yang [République populaire de Chine] ; Kaijian Wu [République populaire de Chine] ; Jing Liu [République populaire de Chine] ; Qun Liu [République populaire de Chine]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2020.
Abstract
Neospora caninum is an obligate intracellular protozoan parasite that infects a wide range of mammalian species and causes spontaneous abortion in cattle. N. caninum is exposed to oxidative stress during its life cycle. Oxidoreductase is crucial for parasite response to the environmental stresses. Glutaredoxins (Grxs) are small oxidoreductases of the thioredoxin family proteins that catalyze thiol-disulfide exchange reactions by utilizing electrons from the tripeptide glutathione (γGlu-Cys-Gly; GSH). Grxs are key elements in redox signaling and cell signal transduction. However, Grxs are an unexplored set of oxidoreductases in N. caninum. Here, we identified two cytoplasm located glutaredoxin domain-containing proteins (NcGrx1 and NcGrx3) in N. caninum. To better understand the functions of these Grx proteins, we generated NcGrx1 and NcGrx3 deficiency and overexpression strains. The deletion or overexpression of NcGrx3 had no significant effect on the growth of N. caninum in vitro and in vivo. NcGrx1 knockout parasites displayed a significant growth defect, which was due to the influence on invasion and egress abilities. Moreover, NcGrx1 deficiency decreased the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) (GSH/GSSG ratio), caused a significant accumulation of hydroxyl radical in parasites, and an increase in apoptotic cells under oxidative stress (H2O2) condition. To determine the cause of growth defects in ΔNcGrx1, we examined the transcription levels of various invasion-egress related genes as measured by qPCR. We found a significant decrease in MIC1, MIC4, and MIC6 genes. Further investigation found that the secretion of MIC1, MIC4, and MIC6 proteins was significantly affected. Collectively, Ncgrx1 is important for microneme protein-mediated parasite growth, and maybe a potential intervention target for the N. caninum.
DOI: 10.3389/fmicb.2020.536044
PubMed: 32983074
PubMed Central: PMC7487798
Affiliations:
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Xingju" uniqKey="Song X" first="Xingju" last="Song">Xingju Song</name><affiliation wicri:level="3"><nlm:affiliation>National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, 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Chine</country><wicri:regionArea>National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Qun" sort="Liu, Qun" uniqKey="Liu Q" first="Qun" last="Liu">Qun Liu</name><affiliation wicri:level="3"><nlm:affiliation>National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Neospora caninum</i> is an obligate intracellular protozoan parasite that infects a wide range of mammalian species and causes spontaneous abortion in cattle. <i>N. caninum</i> is exposed to oxidative stress during its life cycle. Oxidoreductase is crucial for parasite response to the environmental stresses. Glutaredoxins (Grxs) are small oxidoreductases of the thioredoxin family proteins that catalyze thiol-disulfide exchange reactions by utilizing electrons from the tripeptide glutathione (γGlu-Cys-Gly; GSH). Grxs are key elements in redox signaling and cell signal transduction. However, Grxs are an unexplored set of oxidoreductases in <i>N. caninum</i>. Here, we identified two cytoplasm located glutaredoxin domain-containing proteins (NcGrx1 and NcGrx3) in <i>N. caninum</i>. To better understand the functions of these Grx proteins, we generated NcGrx1 and NcGrx3 deficiency and overexpression strains. The deletion or overexpression of NcGrx3 had no significant effect on the growth of <i>N. caninum in vitro</i> and <i>in vivo</i>. NcGrx1 knockout parasites displayed a significant growth defect, which was due to the influence on invasion and egress abilities. Moreover, NcGrx1 deficiency decreased the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) (GSH/GSSG ratio), caused a significant accumulation of hydroxyl radical in parasites, and an increase in apoptotic cells under oxidative stress (H<sub>2</sub>O<sub>2</sub>) condition. To determine the cause of growth defects in ΔNcGrx1, we examined the transcription levels of various invasion-egress related genes as measured by qPCR. We found a significant decrease in MIC1, MIC4, and MIC6 genes. Further investigation found that the secretion of MIC1, MIC4, and MIC6 proteins was significantly affected. Collectively, Ncgrx1 is important for microneme protein-mediated parasite growth, and maybe a potential intervention target for the <i>N. caninum</i>.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32983074</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>Glutaredoxin 1 Deficiency Leads to Microneme Protein-Mediated Growth Defects in <i>Neospora caninum</i>.</ArticleTitle><Pagination><MedlinePgn>536044</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2020.536044</ELocationID><Abstract><AbstractText><i>Neospora caninum</i> is an obligate intracellular protozoan parasite that infects a wide range of mammalian species and causes spontaneous abortion in cattle. <i>N. caninum</i> is exposed to oxidative stress during its life cycle. Oxidoreductase is crucial for parasite response to the environmental stresses. Glutaredoxins (Grxs) are small oxidoreductases of the thioredoxin family proteins that catalyze thiol-disulfide exchange reactions by utilizing electrons from the tripeptide glutathione (γGlu-Cys-Gly; GSH). Grxs are key elements in redox signaling and cell signal transduction. However, Grxs are an unexplored set of oxidoreductases in <i>N. caninum</i>. Here, we identified two cytoplasm located glutaredoxin domain-containing proteins (NcGrx1 and NcGrx3) in <i>N. caninum</i>. To better understand the functions of these Grx proteins, we generated NcGrx1 and NcGrx3 deficiency and overexpression strains. The deletion or overexpression of NcGrx3 had no significant effect on the growth of <i>N. caninum in vitro</i> and <i>in vivo</i>. NcGrx1 knockout parasites displayed a significant growth defect, which was due to the influence on invasion and egress abilities. Moreover, NcGrx1 deficiency decreased the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) (GSH/GSSG ratio), caused a significant accumulation of hydroxyl radical in parasites, and an increase in apoptotic cells under oxidative stress (H<sub>2</sub>O<sub>2</sub>) condition. To determine the cause of growth defects in ΔNcGrx1, we examined the transcription levels of various invasion-egress related genes as measured by qPCR. We found a significant decrease in MIC1, MIC4, and MIC6 genes. Further investigation found that the secretion of MIC1, MIC4, and MIC6 proteins was significantly affected. Collectively, Ncgrx1 is important for microneme protein-mediated parasite growth, and maybe a potential intervention target for the <i>N. caninum</i>.</AbstractText><CopyrightInformation>Copyright © 2020 Song, Yang, Xue, Yang, Wu, Liu and Liu.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Xingju</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xu</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xue</LastName><ForeName>Yangfei</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Congshan</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Kaijian</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Jing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Qun</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Microbiol</MedlineTA><NlmUniqueID>101548977</NlmUniqueID><ISSNLinking>1664-302X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Neospora caninum</Keyword><Keyword MajorTopicYN="N">egression</Keyword><Keyword MajorTopicYN="N">glutaredoxin 1</Keyword><Keyword MajorTopicYN="N">invading</Keyword><Keyword MajorTopicYN="N">microneme proteins</Keyword><Keyword MajorTopicYN="N">reactive oxygen species</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>02</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>08</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>28</Day><Hour>5</Hour><Minute>40</Minute></PubMedPubDate><PubMedPubDate 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IdType="pubmed">30503087</ArticleId></ArticleIdList></Reference><Reference><Citation>Exp Parasitol. 1998 Nov;90(3):277-85</Citation><ArticleIdList><ArticleId IdType="pubmed">9806873</ArticleId></ArticleIdList></Reference><Reference><Citation>Parasitol Res. 2015 Aug;114(8):2893-902</Citation><ArticleIdList><ArticleId IdType="pubmed">25956399</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2014 Jun 17;5:4098</Citation><ArticleIdList><ArticleId IdType="pubmed">24934579</ArticleId></ArticleIdList></Reference><Reference><Citation>Antioxid Redox Signal. 2013 Sep 1;19(7):683-95</Citation><ArticleIdList><ArticleId IdType="pubmed">23256874</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2009 Jan 23;385(3):889-901</Citation><ArticleIdList><ArticleId IdType="pubmed">18992757</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2010 May 20;465(7296):359-62</Citation><ArticleIdList><ArticleId IdType="pubmed">20485436</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Enzymol. 1995;252:283-92</Citation><ArticleIdList><ArticleId IdType="pubmed">7476363</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2008 Nov;1780(11):1304-17</Citation><ArticleIdList><ArticleId IdType="pubmed">18621099</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1997 Jan 3;275(5296):90-4</Citation><ArticleIdList><ArticleId IdType="pubmed">8974401</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2000 May 12;275(19):14346-53</Citation><ArticleIdList><ArticleId IdType="pubmed">10799515</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Microbiol. 2015;69:463-85</Citation><ArticleIdList><ArticleId IdType="pubmed">26332089</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2005 May 6;330(2):604-10</Citation><ArticleIdList><ArticleId IdType="pubmed">15796926</ArticleId></ArticleIdList></Reference><Reference><Citation>Mini Rev Med Chem. 2006 Feb;6(2):163-76</Citation><ArticleIdList><ArticleId IdType="pubmed">16472185</ArticleId></ArticleIdList></Reference><Reference><Citation>Top Companion Anim Med. 2010 Aug;25(3):170-5</Citation><ArticleIdList><ArticleId IdType="pubmed">20937501</ArticleId></ArticleIdList></Reference><Reference><Citation>Parasitol Res. 2019 Jun;118(6):1785-1797</Citation><ArticleIdList><ArticleId IdType="pubmed">31062084</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Cell. 2002 Apr;13(4):1109-21</Citation><ArticleIdList><ArticleId IdType="pubmed">11950925</ArticleId></ArticleIdList></Reference><Reference><Citation>Free Radic Biol Med. 2011 Dec 1;51(11):2108-17</Citation><ArticleIdList><ArticleId IdType="pubmed">21983434</ArticleId></ArticleIdList></Reference><Reference><Citation>Vet Parasitol. 1999 Aug 1;84(3-4):349-67</Citation><ArticleIdList><ArticleId IdType="pubmed">10456423</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2010 Nov 5;285(45):35224-37</Citation><ArticleIdList><ArticleId IdType="pubmed">20826822</ArticleId></ArticleIdList></Reference><Reference><Citation>Exp Parasitol. 1997 Oct;87(2):88-97</Citation><ArticleIdList><ArticleId IdType="pubmed">9326884</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biochem Parasitol. 2016 Mar-Apr;206(1-2):75-83</Citation><ArticleIdList><ArticleId IdType="pubmed">26593282</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2013 Nov 04;4:428</Citation><ArticleIdList><ArticleId IdType="pubmed">24204369</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Med. 2005 Feb 7;201(3):453-63</Citation><ArticleIdList><ArticleId IdType="pubmed">15684324</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Microbiol. 2011 Aug;14(4):422-8</Citation><ArticleIdList><ArticleId IdType="pubmed">21803641</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2002 Nov 29;277(48):46566-75</Citation><ArticleIdList><ArticleId IdType="pubmed">12244106</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Microbiol. 2013 Mar;15(3):795-810</Citation><ArticleIdList><ArticleId IdType="pubmed">22891731</ArticleId></ArticleIdList></Reference><Reference><Citation>mBio. 2015 Sep 15;6(5):e00845-15</Citation><ArticleIdList><ArticleId IdType="pubmed">26374117</ArticleId></ArticleIdList></Reference><Reference><Citation>J Cell Biol. 2001 Feb 5;152(3):563-78</Citation><ArticleIdList><ArticleId IdType="pubmed">11157983</ArticleId></ArticleIdList></Reference><Reference><Citation>Free Radic Biol Med. 2014 Nov;76:208-26</Citation><ArticleIdList><ArticleId IdType="pubmed">25157786</ArticleId></ArticleIdList></Reference><Reference><Citation>Antioxid Redox Signal. 2013 Sep 1;19(7):708-22</Citation><ArticleIdList><ArticleId IdType="pubmed">22978520</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2008 Mar 19;582(6):848-54</Citation><ArticleIdList><ArticleId IdType="pubmed">18275854</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochimie. 2014 Nov;106:56-67</Citation><ArticleIdList><ArticleId IdType="pubmed">25110158</ArticleId></ArticleIdList></Reference><Reference><Citation>Parasit Vectors. 2016 Jun 21;9(1):352</Citation><ArticleIdList><ArticleId IdType="pubmed">27329357</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 2012 Jan;83(2):304-18</Citation><ArticleIdList><ArticleId IdType="pubmed">22151036</ArticleId></ArticleIdList></Reference><Reference><Citation>Vet Parasitol. 2005 Mar 31;128(3-4):231-41</Citation><ArticleIdList><ArticleId IdType="pubmed">15740860</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2017 Nov;15(11):645-660</Citation><ArticleIdList><ArticleId IdType="pubmed">28867819</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Microbiol. 2009 Aug;12(4):415-21</Citation><ArticleIdList><ArticleId IdType="pubmed">19616990</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Med Microbiol. 2012 Oct;302(4-5):187-94</Citation><ArticleIdList><ArticleId IdType="pubmed">22939033</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2009 Jan 23;323(5913):530-3</Citation><ArticleIdList><ArticleId IdType="pubmed">19095897</ArticleId></ArticleIdList></Reference><Reference><Citation>Free Radic Biol Med. 2017 Mar;104:104-117</Citation><ArticleIdList><ArticleId IdType="pubmed">28062360</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry (Mosc). 2014 Dec;79(13):1562-83</Citation><ArticleIdList><ArticleId IdType="pubmed">25749165</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biochem Parasitol. 2015 Dec;204(2):93-105</Citation><ArticleIdList><ArticleId IdType="pubmed">26854591</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 2001 Aug;41(3):537-47</Citation><ArticleIdList><ArticleId IdType="pubmed">11532123</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2001 Feb 9;276(6):4119-27</Citation><ArticleIdList><ArticleId IdType="pubmed">11053441</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2017 Apr 04;8:14835</Citation><ArticleIdList><ArticleId IdType="pubmed">28374771</ArticleId></ArticleIdList></Reference><Reference><Citation>Parasitol Res. 2009 Aug;105(2):441-5</Citation><ArticleIdList><ArticleId IdType="pubmed">19308454</ArticleId></ArticleIdList></Reference><Reference><Citation>Antioxid Redox Signal. 2012 Dec 15;17(12):1748-63</Citation><ArticleIdList><ArticleId IdType="pubmed">22530666</ArticleId></ArticleIdList></Reference><Reference><Citation>Parasit Vectors. 2015 Jul 22;8:384</Citation><ArticleIdList><ArticleId IdType="pubmed">26194005</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Microbiol. 2010 Dec;12(12):1792-808</Citation><ArticleIdList><ArticleId IdType="pubmed">20678172</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteome Res. 2018 Mar 2;17(3):1091-1100</Citation><ArticleIdList><ArticleId IdType="pubmed">29356545</ArticleId></ArticleIdList></Reference><Reference><Citation>Redox Biol. 2018 May;15:532-547</Citation><ArticleIdList><ArticleId IdType="pubmed">29413965</ArticleId></ArticleIdList></Reference><Reference><Citation>Exp Biol Med (Maywood). 2001 Mar;226(3):229-36</Citation><ArticleIdList><ArticleId IdType="pubmed">11361042</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13227-32</Citation><ArticleIdList><ArticleId IdType="pubmed">15328416</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de 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Yangfei" uniqKey="Xue Y" first="Yangfei" last="Xue">Yangfei Xue</name><name sortKey="Xue, Yangfei" sort="Xue, Yangfei" uniqKey="Xue Y" first="Yangfei" last="Xue">Yangfei Xue</name><name sortKey="Yang, Congshan" sort="Yang, Congshan" uniqKey="Yang C" first="Congshan" last="Yang">Congshan Yang</name><name sortKey="Yang, Congshan" sort="Yang, Congshan" uniqKey="Yang C" first="Congshan" last="Yang">Congshan Yang</name><name sortKey="Yang, Xu" sort="Yang, Xu" uniqKey="Yang X" first="Xu" last="Yang">Xu Yang</name><name sortKey="Yang, Xu" sort="Yang, Xu" uniqKey="Yang X" first="Xu" last="Yang">Xu Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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